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Killed 4 days later. The protective effect was assessed by counting the cysticercoids which had developed in the intestinal villi by day 9, 4 days after challenge. Monoclonal antibodies were intraperitoneal injected at 0.5 mg on 2 hours before and 48 hours after primary infection. These antibodies are neutralizing monoclonal antibodies against mouse CD80, CD86, ICOS L and CD40 L. Treatment of neither antibodies on protective immunity failed to suppress acquired resistance to H. nana re-infection. CD28 knock out mice were carried out as above infection. In this knock out mice, protective immunity also failed to suppress acquired resistance. The present results suggest that protective immunity against H. nana oncosphere is establised independent to known costimulatory molecules and naltrexone!
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